FIG. 2.

Hamp1, Id1, and Smad7 expression, and Bmp6/Smad signaling in Hfe^−/−, Hjv^−/−, and Hfe^−/−Hjv^−/− double-knockout mice. Hepatic (A) Hamp1, (B) Id1, (C) Smad7, and (D) Bmp6 mRNA levels were measured in 8-week-old wild-type (eight male, eight female), Hfe^−/− (eight male, eight female), Hjv^−/− (eight male, eight female), and Hjv^−/−Hfe^−/− (eight male, eight female) mice that were fed a standard diet; the mRNA levels were normalized to β-actin mRNA and are expressed relative to the mean value of wild-type males. The (E) Hamp1/Bmp6, (F) Id1/Bmp6, and (G) Smad7/Bmp6 ratios were calculated for each mouse and relative to the mean value of wild-type males. Statistical analyses were conducted by using log-transformed values to meet homogenous variances, and then all data were compared using an ANOVA with Tukey's test. Labeled without a common letter (a, b, c, d, or e) indicates significant difference among tested groups (p<0.05).