FIG. 5.

Hjv^−/− and Hjv^−/−Hfe^−/− mice have an attenuated hepcidin response to acute iron treatment. Eight-week-old wild-type, Hfe^−/−, Hjv^−/−, and Hjv^−/−Hfe^−/− mice were given FeCl₃ (“Iron”; n=8 mice/group) or distilled water (“Mock”; n=8 mice/group) by oral gavage; after 4 h, (A) Bmp6, (B) Hamp1, (C) Id1, (D) Smad7 mRNA, and (G) P-Smad1/5/8 levels were measured. Hepatic (E) Hamp1 mRNA levels in Hfe^−/−, Hjv^−/−, and Hfe^−/−Hjv^−/− mice that were fed an iron-deficient diet were detected, and (F) the hepatic Hamp1/Bmp6 ratio was calculated for each mouse. Hepatic Bmp6, Hamp1, Id1, and Smad7 mRNA was normalized to β-actin and expressed relative to the mean value of each genotype's respective mock group. Hepatic P-Smad1/5/8 levels were quantified, normalized to Smad1, and expressed relative to the mean value of each genotype's respective mock group. The data in (A–D) and (G) were analyzed using the Student's t-test; *p<0.05 versus the respective mock group. The data in (E, F) were compared using an ANOVA with Tukey's test. Labeled without a common letter (a, b) indicates significant difference among tested groups (p<0.05).