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Interactions of Class 3 NESs with CRM1. Binding between recombinant purified GST-NESs and CRM1 is shown by pull-down binding assays. Eleven GST-Class 3 NESs were immobilized and incubated with CRM1 in the presence and absence of RanGTP. Bound proteins were resolved with SDS-PAGE and visualized by Coomassie staining. All 11 NESs conform only to Class 3 patterns. (a) Class 3 NESs that bind CRM1. (b) Class 3 NESs that do not bind CRM1
