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. 1994 Jun 21;91(13):5913–5917. doi: 10.1073/pnas.91.13.5913

Human immunodeficiency virus integrase directs integration to sites of severe DNA distortion within the nucleosome core.

D Pruss 1, F D Bushman 1, A P Wolffe 1
PMCID: PMC44107  PMID: 8016088

Abstract

We have examined the consequences of DNA distortion and specific histone-DNA contacts within the nucleosome for integration mediated by the human immunodeficiency virus (HIV)-encoded integrase enzyme. We find that sites of high-frequency integration cluster in the most severely deformed, kinked DNA regions within the nucleosome core. This may reflect either a preference for a wide major groove for association of the integrase or a requirement for target DNA distortion in the DNA strand transfer mechanism. Both the distortion and folding of the target DNA through packaging into nucleosomes may influence the selection of HIV integration sites within the chromosome.

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Selected References

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