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. Author manuscript; available in PMC: 2015 Apr 27.
Published in final edited form as: Nat Methods. 2014 Aug;11(8):801–807. doi: 10.1038/nmeth.3027

Figure 2. Large-scale mutational data illustrate how protein sequence impacts function.

Figure 2

A hypothetical sequence–function heat map is shown for a 25 amino acid long portion of a protein, illustrating the functional consequences of making every single amino acid mutation at every position. Positions are indicated numerically, and each mutation is indicated by its single letter code. The color of each element of the heat map illustrates the functional score of the indicated mutation