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. Author manuscript; available in PMC: 2015 Apr 27.
Published in final edited form as: Nat Methods. 2014 Aug;11(8):801–807. doi: 10.1038/nmeth.3027

Figure 3. Deep mutational scanning in sensitized backgrounds as a strategy for uncovering protein features.

Figure 3

Hypothetical sequence-function heat maps collected under different conditions are shown. Once a deep mutational scan has been performed, it can be repeated in a sensitized background, which can be created by altering the cellular or chemical environment in which the scan is conducted, as indicated. The difference in functional effect for a particular mutation in a sensitized background could reveal the importance of an amino acid at a given position for the process under study.