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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: J Immunol. 2015 Jan 12;194(4):1945–1953. doi: 10.4049/jimmunol.1402408

Figure 2. Acquisition of m157 by NK cells is independent of the Ly49H receptor.

Figure 2

(A) IL-2 stimulated NK cells (LAKs) from B6.BxD8 mice were incubated with RMA (□) or RMAm157 (●) and assessed by flow cytometry for the percentage of NK cells that were positive for m157 on their cell surface at various time points (n=3 for each time point). (B) B6.BxD8 LAKs were incubated with RMAm157 cells for 2 hours in the presence (n=3) or absence (n=3) of PI-PLC. The percentage of LAKs expressing m157 was assessed by flow cytometry (C) B6.BxD8 LAKs were incubated for 2 hours with RMA or RMAm157 cells in the presence (n=4–6) or absence (n=4–6) of a transwell. The percentage of LAK cells expressing m157 was assessed by flow cytometry. All data are presented as the mean+/− SEM. *p<0.0005.