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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: J Immunol. 2015 Jan 12;194(4):1945–1953. doi: 10.4049/jimmunol.1402408

Figure 5. The m157-dressed NK cells are defective at killing m157-expressing targets.

Figure 5

WT IL-2 stimulated NK cells (LAKs) were incubated with RMA or RMAm157 cells and then sorted to obtain “dressed” LAKs. The sorted LAKs were used as effector cells at 3:1 and 10:1 E:T ratios. (A) Representative histogram to evaluate killing of RMAm157 target cells based on propidium iodide (PI) incorporation assessed by flow cytometry. The numbers in the histogram are the percentage of cells present in the PI+ and PI− gates. (B) Percentage of RMAm157 target cells killed at two different E:T ratios. Effector cells were sorted RMA- “dressed” LAKs (RMAd; black, n=6), sorted RMAm157-“dressed” LAKs (m157d; white, n=12) or unsorted B6.BxD8 LAKs (B6.BxD8; hatched, n=9). All data are presented as the mean+/− SEM. *p<0.0005.