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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: J Immunol. 2015 Jan 12;194(4):1945–1953. doi: 10.4049/jimmunol.1402408

Figure 6. Expression of the Ly49H receptor is required to induce NK cell hyporesponsiveness.

Figure 6

(A) Representative histograms displaying m157 expression, as assessed by flow cytometry, on donor NK cells from either B6.BxD8, WT or DAP12ki mice injected into non-Tg recipient (tinted histogram) or into m157-Tg recipient mice (open histogram with solid line). The expression of m157 on NK cells from m157-Tg recipient mice is also shown (open histogram with dashed line). (B) B6.BxD8, WT, or DAP12ki splenocytes were injected into WT (black) or m157-Tg (white) recipient mice (n=5–6). Splenocytes were isolated at 2–3 days post transfer and assessed for IFN-γ production by donor NK cells following stimulation through the NK1.1 receptor. All data are presented as the mean+/− SEM. *p<0.02, ns=not significant. (C) WT or DAP12ki splenocytes were injected into WT or m157-Tg recipient mice (n=5–6). Splenocytes were isolated at 2–3 days post transfer and assessed for IFN-γ production by donor Ly49H+ NK cells (black) or donor Ly49H− NK cells (white) following stimulation through the NK1.1 receptor. All data are presented as the mean+/− SEM.*p<0.0005, **p<0.005, ns=not significant.