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(a) Experimental setup for pulsed focused ultrasound (pFUS) treatment of the mouse calf muscle, as described in the text. Treatments were performed in a heated water bath to ensure good acoustic coupling, (b) The leg is rotated 90°, and the positioning of the ultrasound focus is indicated. The long axis of the transducer focus lies completely within the muscle midbelly without approaching the tibia. The region of micro-computed tomography analysis is noted in the proximal tibia.
