Fig. 1.
Principle of the β-lactamase assay for quantifying virus–cell fusion. (A) A diagram of the Förster resonance energy transfer (FRET)-based BlaM assay. (B) Images of cells loaded with the CCF4-AM BlaM substrate (left panel) and loaded cells after inoculation with HXB2 pseudoviruses bearing the BlaM-Vpr chimera (right).