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Assay development for a 384-well high-throughput screening (HTS) format. Fusion of HXB2.BlaM-Vpr pseudoviruses with TZM-bl cells cultured in 384-well plates. (A) The BlaM assay yields robust values for signal-to-background (S/B) and Z′ with minimal day-to-day variations. (B) The specificity of the BlaM signal was tested using a known inhibitor of HIV-1 fusion, C52L, added at 1 μM. The data are mean with standard deviation (SD) of four replicates.
