Fig. 6.

P2X1 receptor inhibitor blocks cell–cell fusion mediated by HIV-1 Env glycoprotein. HeLa cells constitutively expressing HIV-1 ADA Env and Tat were overlaid onto the indicator TZM-bl cells in the presence or absence of inhibitors, and cell–cell fusion was triggered by shifting to 37°C. The extent of cell–cell fusion was measured by the Tat-dependent luciferase expression in TZM-bl cells. Both NF279 (10 μM) and the fusion inhibitor C52L (1 μM) blocked HIV-1 Env-mediated fusion. The data are mean and SD from one experiment performed in triplicate.