FIG. 2.

Myricetin effects on the Ca²⁺-dependent exocytotic component of 4-AP-evoked glutamate release. (A) Ca²⁺-independent release was assayed by omitting CaCl₂ and adding 300 μM EGTA 10 min before depolarization and was evoked by 1 mM 4-AP under control conditions or in the presence of 10 μM dl-threo-beta-benzyl-oxyaspartate (dl-TBOA) or 30 μM myricetin, added 10 min before the addition of 4-AP. Glutamate release was evoked by 1 mM 4-AP in the absence (control) or presence of 10 μM DL-TBOA (B) or 0.1 μM bafilomycin A1 (C) added 10 min before the addition of 30 μM myricetin. (D) Quantitative comparison of the extent of glutamate release by 1 mM 4-AP in the absence and presence of 30 μM myricetin, and absence and presence of 50 μM BAPTA, 10 μM dl-TBOA, or 0.1 μM bafilomycin A1. Results are mean±SEM of 5–7 independent experiments. ***P<.001 versus control, **P<.01 versus control, ^#P<.05 versus the dl-TBOA-treated group.