Table 1.
Strains and plasmids used in this study
| Strains or plasmids | Relevant characteristics | Source |
|---|---|---|
| Plasmids | ||
| pKOR1 | E. coli– S. aureus shuttle vector, ori(Ts) inducible, secY antisense counterselection, Ampr Cmr | Dr Bae |
| pMOE 399 | pKOR1: Δ msa operon deletion construct | This study |
| pCN34 | pT181-based low copy number E. coli–Staphylococcal shuttle vector | NARSA |
| pMOE 403 | pCN34-msaABCR operon :: msaABCR operon complement | This study |
| pCN58 | pT181-based E.coli–staphylococcal shuttle vector that contains promoterless LuxAB as a reporter gene for promoter-gene fusion. | NARSA |
| pMOE 481 | pCN58-Promoter aur | This study |
| pMOE 482 | pCN58-Promoter ssp | This study |
| pMOE 483 | pCN58-Promoter scp | This study |
| pMOE 498 | pCN58-Promoter spl | This study |
| pMOE 501 | pCN58-Promoter | This study |
| pMOE 448 | E. coli pCN51 vector with pCAD inducible promoter | This study |
| Strains | ||
| RN4220 | Restriction deficient mutant of 8325–4 | NARSA |
| M. luteus | ATCC No. 4698 | Sigma–Aldrich |
| LAC | CA-MRSA USA300 strains | Dr Shaw |
| MOE 401 | LAC :: Δ msaABCR deletion mutant | This study |
| MOE 404 | LAC 401 :: pMOE403 :: msaABCR complement into msaABCR deletion mutant | This study |
| MOE 401/pCN34-vector control | This study | |
| MOE 508 | LAC 13C (Δatl) | Dr Jeffrey Bose /K. Bayles |
| MOE 458 | LAC/proteases | Dr Mark Smeltzer |
| MOE 512 | msaABCR/atl mutant | This study |
| MOE 466 | msaABCR/protease mutant | This study |
| msaABCR complement into msaABCR/atl mutant | This study | |
| MOE 467 | msaABCR complement into msaABCR/protease mutant | This study |
| MOE 535 | LAC :: Δ sarA:kan insertional mutant | This study |
| MOE 532 | LAC :: Δ msaABCR deletion/sarA:kan insertional mutant | This study |
| MOE 533 | msaABCR/sarA:kan mutant with pCN51-sarA vector | This study |
| MOE 536 | sarA:kan mutant with pCN51-sarA vector | This study |
| MOE 545 | msaABCR deletion mutant with pCN51-sarA vector | This study |
| MOE 645 | LAC :: pCN58-Promoteraur | This study |
| MOE 646 | LAC :: pCN58-Promoter scp | This study |
| MOE 647 | LAC :: pCN58-Promoter spl | This study |
| MOE 648 | LAC :: pCN58-Promoter ssp | This study |
| MOE 649 | MOE 401 :: pCN58-Promoter aur | This study |
| MOE 650 | MOE 401 :: pCN58-Promoterscp | This study |
| MOE 651 | MOE 401 :: pCN58-Promoterspl | This study |
| MOE 652 | MOE 401 :: pCN58-Promoterssp | This study |
| MOE 495 | LAC :: pCN58-vector control | This study |