Fig. 8.

Interaction between Dom7–15 and Dom14–15 constructs and the lysosomal enzyme GAA modified with either M6P (monoester) or M6P-GlcNAc (diester) at the pH of the Golgi. Representative sensorgrams collected at pH 6.5 showing the interaction of increasing concentrations of (A) Dom14–15 (120, 200, 400, 1000, 5000 and 20000 nM) and (B) Dom7–15 (5, 10, 15, 20, 40, 80, 120, 200, 400, and 1000 nM) with GAA monoester and GAA diester coupled CM5 surfaces. Response units (RU) are shown. This figure is available in black and white in print and in colour at Glycobiology online.