Table II.

Kinetic data obtained from SPR analyses at pH 6.5

Construct					KD (nM)
	ka1 (M−1s−1 × 103)		kd1 (s−1 × 10−3)		Monoester		Diester
	Monoester	Diester	Monoester	Diester	kd1/ka1	Steadystate	kd1/ka1	Steadystate
D7–15	101	53	7.42	0.0706	74	97	1300	926
D14–15	6.4	4.6	0.11	0.14	17000	9600	29000	45000
					KD2 (mM)
	ka2 (M−1s−1 × 10−3)		kd2 (s−1 × 10−3)		kd2/ka2
Construct	Monoester	Diester	Monoester	Diester	Monoester	Diester
D7–15	0.69	7.1	0.13	5.7	0.19	0.81
D14–15	28	3.8	17.4	9.8	0.62	2.6

Kinetic and steady state affinity values determined from SPR sensorgrams obtained with GAA monoester and GAA diester immobilized on sensor chip surfaces. Purified recombinant protein was injected as the free analyte. Upper table reports kinetic parameters of the major component obtained from fitting sensorgrams to a two-state model with conformational change. The lower table reports kinetic parameters for the minor component with K_D values reported in mM.