Fig. 3.

Injection of tmem165 directed antisense morpholinos into zebrafish embryos reduces transcript abundance resulting in multiple gross phenotypes. (A) Schematic representation of tmem165 gene. The positions of TB and SB MOs are indicated. Arrows indicate the position of primers used to assess transcript abundance (see the Materials and Methods section). (B) RT–PCR of 4 dpf embryos after injection of indicated concentrations of SB MO. (C) Schematic representation of the altered products that result following injection of the SB MO. (D) Injection of either 1.5 ng of the SB MO or 1 ng of the TB MO resulted in shorter embryos with small misshapen heads. TB morphants are shown. Arrowheads indicated abnormalities in the ventral jaw structures of tmem165 morphants. Co-injection of tmem165 mRNA rescued these phenotypes. (E) Body length measurements of 4 dpf embryos after injection of TB MO indicate a reduction in size that was rescued by co-injection of wild-type mRNA. n = 3 experiments each containing 25 embryos. *P < 0.05, **P < 0.01 and ***P < 0.001 (Student's t test). Error bars represent the S.E.M. of three independent biological samples. This figure is available in black and white in print and in colour at Glycobiology online.