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. Author manuscript; available in PMC: 2015 Apr 27.
Published in final edited form as: Nat Protoc. 2012 May;7(5):921–934. doi: 10.1038/nprot.2012.020

Figure 4.

Figure 4

Sample chambers are made by putting a quartz slide (1) and a glass coverslip (3) together with pieces of double-sided tape (2) and sealing with epoxy. The holes on the quartz slide are used for the inlet and outlet of solution exchange through each channel with a volume of ~10 µl.