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. Author manuscript; available in PMC: 2015 Apr 27.
Published in final edited form as: Nat Protoc. 2012 May;7(5):921–934. doi: 10.1038/nprot.2012.020
t-vesicle v-vesicle
t-vesicles (10 mM) 10 µl
v-vesicles (10 mM) 10 µl
Sso1pHT proteins (20 µM) 50 µl
Snc2p proteins (20 µM) 50 µl
10% OG 0.9 µl 0.9 µl