Table 3.
Target mRNA expression in miRNA transfected colon cancer cell lines
| Transfected MicroRNA |
Average Fold Change of 3 different transfections when compared to Negative Control in HT-29 cell line |
Average Fold Change of 3 different transfections when compared to Negative Control in HCT-116 cell line |
Target mRNA |
Fold Change in HT-29 Cells (p-value)* |
Fold Change in HCT-116 Cells (p-value)* |
|---|---|---|---|---|---|
| Let-7e | 490× | 560× | E2F1 | 0.82 (0.0248) | 0.63 (0.0248) |
| miR-17 | 57× | 86× | SIRT1 | 1.10 (0.138) | 0.88 (0.138) |
| PCAF | 1.12 (0.063) | 1.38 (0.0248) | |||
| TP53 | 1.12 (0.138) | 1.06 (0.256) | |||
| E2F1 | 0.72 (0.049) | 0.89 (0.138) | |||
| miR-122 | 180× | 220× | CCNG1 | 0.72 (0.0248) | 0.55 (0.0248) |
*
p-value calculated using the Mann-Whitney U-test, p<0.05 is significant (Italicized). Fold Change <1 is a down-regulation while fold change >1 is an up-regulation