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. 2014 Jul 21;4(4):463–473. doi: 10.1021/sb500252a

Figure 4.

Figure 4

In vivo colonization of tumors with low doses of E. coli expressing synthetic adhesins. (a) Bacterial colonization of HeLa-GFP-tm or HeLa tumors, as indicated on top, by the following E. coli strains: wild type K-12 (MG1655), EcM1luxSAtir (SAtir), and EcM1luxSAgfp (SAgfp). Each bacterial strain was intravenously administered (1 × 105 CFU/mouse) to tumor-bearing mice (experimental groups n = 9) and 4-days postadministration the number of CFU in each tumor was determined. Each circle in the graph represents the CFU determined per gram of tumor (Log10 CFU/g) for each animal in the different experimental groups. The ratio of colonized tumors in each group is shown at the bottom along with the statistical analyses between groups connected with lines. Two-tailed P values of Fisher’s exact test are indicated with one asterisk (*) when P < 0.05 or two asterisks (**) when P = 0.015. On the right, bioluminescence live imaging of HeLa-GFP-tm tumor-bearing mouse infected with 1 × 105 CFU of EcM1luxSAtir (left image) or EcM1luxSAgfp (right image). Images are overlays of photographic white-light and bioluminescence signals from a representative tumor-bearing mouse infected with the strains, as indicated on top. The intensities of the bioluminescence signals are represented in pseudocolor according to the scale bar. (b) Graphs showing bacterial titers in livers (left) and spleens (right) from those animals with a HeLa-GFP-tm tumor colonized in panel a by wild-type K-12 (MG1655), EcM1luxSAtir (SAtir), or EcM1luxSAgfp (SAgfp) strains. Each circle in the graph represents the CFU determined per gram of tissue (log10 CFU/g).