Figure 6.

ATRX-Mediated Deposition of H3.3 Maintains Heterochromatic Profiles during Transcription

(A) Schematic depiction of a gene containing a gene body CGI. Heterochromatic modifications such as H3K9me3 silence the internal CGI to prevent cryptic transcription.

(B) Progression of RNA Pol II during transcription disrupts heterochromatin-modified nucleosomes at the internal CGI.

(C) ATRX/Daxx mediate deposition of replacement H3.3 at the heterochromatic CGI and facilitate maintenance of chromatin modifications at this site.

(D) Loss of ATRX leads to failure of H3.3 deposition, loss of chromatin modifications, and aberrant transcription.