Figure 2.

mNET-Seq with Different Phospho-CTD Modifications

(A) Diagram showing different Pol II antibody epitopes on CTD (Stasevich et al., 2014).

(B) Specificity of Pol II phosphorylation released from chromatin following MNase treatment with indicated Pol II antibodies.

(C) Meta-analyses of mNET-seq/unph+ph on TSS and TES of pA⁺ protein-coding genes (left) and histone genes (right). Read density (FPKM) of mNET-seq data were plotted around TSS (±0.5 kb) and TES (−0.5 k∼+3 kb). Data on pA⁺ and histone genes are represented as mean ± SEM. mNET-seq sense strand, blue; antisense strand, red.

(D) Meta-analyses of mNET-seq on TSS and TES of pA⁺ protein-coding genes. Ratio of read density (FPKM) of indicated mNET-seq data to mNET-seq/unph+ph data was plotted around TSS (±0.5 kb) and TES (−0.5 k∼+3 kb). unph, dark gray; S2P, blue; S5P, red. Line and shading represent mean ± SEM for each bin.

(E and F) mNET-seq profiles over TSS of TARDBP (E) and TES of CDK1 (F). Read density, read per 10⁸ sequences.