Figure 7.

Promoter-Associated RNA Turnover Regulated by Termination Factors

(A) Meta-analysis of mNET-seq with indicated Pol II antibodies over TSS regions (−0.5k∼+0.5 kb) from siLuc (dark gray) and siCPSF73 (red) treated HeLa cells (left).

(B) Meta-analyses of mNET-seq/S2P following knockdown of CstF64+CstF64t (blue) and Xrn2 (green) at TSS (left).

(C) mNET-seq of FUS with indicated Pol II antibodies and ChrRNA-seq from siLuc (dark gray) and siCPSF73 (red) treated HeLa cells. Increased mNET-seq/S2P signals following depletion of CPSF73 are denoted by blue arrows.

(D) mNET-seq/S2P maps with indicated knockdowns around TSS of SLC30A6 on both mRNA and PROMPT strands.

(E) Model showing effects of CPA and Xrn2 at TSS. S2P Pol II-CPA complex (red circle) cleaves TSS-associated nascent RNA, and Xrn2 (purple) degrades cleaved RNA from 5′ end to 3′ end over a region of 250 bp from TSS.

For (A) and (B), line and shading represent mean ± SEM for each bin.