Figure S2.

Specificity of Pol II Antibodies, Related to Figure 2

(A) ELISA assay (right) was performed with indicated CTD heptapeptides (left) and Pol II antibodies (right).

(B) Pol II precipitated from cell extracts with indicated antibodies detected by western blot using each antibody.

(C) Pol II ChIP was conducted with indicated Pol II antibodies on GAPDH, IST1, and MYC. Positions of primer sets and PAS are shown by red bars and green triangles, respectively. TSS denoted by black arrow.

(D) meta-analyses of mNET-seq on 5′ end (−0.5 k∼+0.5 kb from TSS) and 3′end (−0.5 k∼+3kb from TES) of histone gene. 8WG16 (unph), dark gray; CMA602 (S2P), blue; CMA603 (S5P), red; n = 20. Line and shading represent mean ± SEM for each bin.