Figure S4.

mNET-seq Profiles for PKM Alternative Splicing after PTBP1 Depletion, Related to Figure 4

(A) PKM exons 8–11 are illustrated. Exon 9 (green) and exon 10 (orange) are mutually exclusive. PCR primers indicated as black triangles. RT-PCR products were digested with indicated exon-specific restriction enzyme (NcoI or PstI).

(B) mNET-seq data around mutually exclusive exons 9 and 10 of PKM. mNET-seq/S5P signals at 3′ end of exon 9 and exon 10 are shown by green and orange arrows, respectively. Transcription direction, black arrow.

(C) Western blot of PTBP1 and tubulin from siPTBP1-treated HeLa cells.

(D) PKM RT-PCR products from PTBP1-depleted HeLa nuclear RNA were digested by NcoI.

(E) mNET-seq/S5P data over mutually exclusive exons 9 and 10 of PKM from siLuc and siPTBP1-treated HeLa cells (top), followed by expanded view around 5′SS of introns 10 and 11. S5P-peaks at 3′ ends of exons, orange asterisks. Transcription direction, black arrows.