Skip to main content
. 2015 Apr 27;10(4):e0125701. doi: 10.1371/journal.pone.0125701

Fig 1. Schematic presentation of the nuclease-linked fluorescence oligonucleotide assay (NLFOA).

Fig 1

(A) In the conventional ELISA, the capture antibody-antigen complex binds to one primary antibody and subsequently to one enzyme-conjugated second antibody. The enzyme acts on the substrate to generate colored substrate products. The reaction is detected by measuring absorbance at a certain wavelength of the chemical reaction. (B) In NLFOA, the capture antibody-antigen complex binds to one biotinylated primary antibody, then to the nanoparticle-streptavidin complex containing multiple copies of streptavidins, and finally to multiple copies of biotinylated TurboNuclease. The fluorescent labeled oliogonucleotide substrate (FLOS), which does not emit fluorescence due to the Iowa Black FQ (quencher) at the other end of the oligo, can generate fluorescence after digestion by TurboNuclease.