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. 2015 Apr 27;10(4):e0125701. doi: 10.1371/journal.pone.0125701

Fig 5. Optimization of the components in NLFOA.

Fig 5

The NLFOA conditions were optimized for (A) nanoparticle-streptavidin, (B) bio-TurboNuclease, (C) FLOS and (D) diameters of the nanoparticles. Both nanoparticle-streptavidin and bio-TurboNuclease were prepared at 1:10 serial dilutions between 10–8 -10-13 M. The FLOS oligos were prepared at 1:2 serial dilutions between 400–3.1 ×10–8 M. Four different diameter sizes (13, 30, 40 and 50 nm) were analyzed for nanoparticles. Negative control (NC) contained all components except what was to be optimized. Each of the component was analyzed in five repeats and were performed in two independent experiments. Means ± SD are shown.