Table 1.
A summary of subjects and RNA results of each neuron-type, matched for age, gender, and postmortem interval (PMI)
| ID | Diagnosis | Age | Gender | PMI | Cell number | A260/A280 | Amount of RNA (μg) | Assaycase |
|---|---|---|---|---|---|---|---|---|
| PYR1 | Control | 22 | M | 21.47 | 530 | 2.37 | 0.004 (69.3) | Microarray/qRT-PCR |
| PYR2 | Control | 75 | M | 20.25 | 520 | 2.44 | 0.014 (54.3) | Microarray/qRT-PCR |
| PYR3 | Control | 58 | F | 21.08 | 680 | 2.39 | 0.007 (50.1) | Microarray/qRT-PCR |
| PYR4 | Control | 71 | F | 20.50 | 530 | 2.47 | 0.012 (39.6) | Microarray/qRT-PCR |
| PYR5 | Schizophrenia | 36 | M | 17.97 | 530 | 2.44 | 0.001 (62.1) | Microarray/qRT-PCR |
| PYR6 | Schizophrenia | 62 | M | 10.75 | 630 | 2.34 | 0.002 (75.9) | Microarray/qRT-PCR |
| PYR7 | Schizophrenia | 67 | F | 21.80 | 540 | 2.52 | 0.002 (62.1) | Microarray/qRT-PCR |
| PYR8 | Schizophrenia | 88 | F | 13.33 | 550 | 2.47 | 0.002 (52.2) | Microarray/qRT-PCR |
| GABA1 | Control | 61 | M | 17.00 | 312 | 2.46 | (47.1) | Microarray/qRT-PCR |
| GABA2 | Control | 22 | M | 21.47 | 295 | 2.44 | (47.4) | Microarray/qRT-PCR |
| GABA3 | Control | 90 | F | 12.66 | 325 | 2.47 | (26.1) | Microarray/qRT-PCR |
| GABA4 | Control | 79 | F | 15.00 | 350 | 2.56 | (31.2) | Microarray/qRT-PCR |
| GABA5 | Schizophrenia | 56 | M | 21.83 | 712 | 2.48 | (22.8) | Microarray/qRT-PCR |
| GABA6 | Schizophrenia | 36 | M | 17.97 | 325 | 2.52 | (24.9) | Microarray/qRT-PCR |
| GABA7 | Schizophrenia | 93 | F | 6.92 | 359 | 2.59 | (25.8) | Microarray/qRT-PCR |
| GABA8 | Schizophrenia | 55 | F | 22.00 | 400 | 2.53 | (24.3) | Microarray/qRT-PCR |
| DA1 | Control | 79 | M | 20.92 | 360 | 1.69 | 0.17 | Microarray/qRT-PCR |
| DA2 | Control | 78 | M | 21.75 | 390 | 1.73 | 0.22 | Microarray/qRT-PCR |
| DA3 | Control | 72 | F | 18.25 | 300 | 1.58 | 0.19 | Microarray/qRT-PCR |
| DA4 | Control | 74 | F | 12.17 | 300 | 1.73 | 0.18 | Microarray/qRT-PCR |
| DA5 | PD | 79 | M | 23.42 | 350 | 1.73 | 0.51 | Microarray/qRT-PCR |
| DA6 | PD | 72 | M | 26.25 | 330 | 1.78 | 0.76 | Microarray/qRT-PCR |
| DA7 | PD | 81 | F | 22.75 | 300 | 1.44 | 0.27 | Microarray/qRT-PCR |
| DA8 | PD | 73 | F | 20.97 | 1300 | 1.82 | 0.68 | Microarray/qRT-PCR |
In all three neuron-types, four representative cases per treatment group or controls are listed. Assay represents the downstream processing used to analyze gene expression. The A260/A280 column contains sample purity ratios for postamplified mRNA for pyramidal and GABAergic neurons, but preamplified total RNA for the dopamine neurons. In the RNA amounts column, the numbers in brackets represent total amount of postamplified mRNA. No measurable preamplified RNA was detected for the GABA samples. DA dopaminergic neurons, GABA parvalbumin-expressing, γ-aminobutyric acid neuron, PD Parkinson disease, Pyr pyramidal neurons