Table 2. List of Differentially Expressed CF Peptides between Healthy Control Samples and Pancreatic Cancer or Chronic Pancreatitisa.
accession no. | protein name | CF peptideb | N/SR | CP/SR | PC/SR |
---|---|---|---|---|---|
Q86TH1 | ADAMTS-like protein 2 | DFTLn*ETVNSIFAQGAPR | 0.71 ± 0.26 | 1.15 ± 0.37c | 1.01 ± 0.28c |
P43652 | Afamin | DIENFn*STQK | 0.54 ± 0.33 | 1.20 ± 0.67c | 1.28 ± 0.85c |
YAEDKFn*ETTEK | 0.40 ± 0.23 | 1.29 ± 0.85c | 1.42 ± 1.09c | ||
P02765 | Alpha-2-HS-glycoprotein | AALAAFNAQNn*GSNFQLEEISR | 0.82 ± 0.26 | 1.43 ± 0.53c | 1.14 ± 0.16c |
P01008 | Antithrombin-III | SLTFn*ETYQDISELVYGAK | 0.77 ± 0.28 | 1.30 ± 0.75 | 1.23 ± 0.41c |
AAINKWVSn*KTEGR | 0.68 ± 0.40 | 1.41 ± 0.69c | 1.17 ± 0.52c | ||
LGACn*DTLQQLMEVFK | 0.82 ± 0.43 | 1.19 ± 0.58 | 1.14 ± 0.33c | ||
P02749 | Beta-2-glycoprotein 1 | DTAVFECLPQHAMFGn*DTITCTTHGn*WTK | 0.60 ± 0.33 | 1.42 ± 0.87c | 1.19 ± 0.67c |
LGn*WSAMPSCK | 0.68 ± 0.44 | 1.16 ± 0.47c | 1.22 ± 0.67c | ||
VYKPSAGn*NSLYR | 0.67 ± 0.34 | 1.55 ± 1.21c | 1.18 ± 0.71c | ||
P20851 | C4b-binding protein beta chain | EWDn*TTTECR | 0.59 ± 0.26 | 1.13 ± 0.54c | 1.12 ± 0.63c |
P07339 | Cathepsin D light chain | GSLSYLn*VTR | 0.81 ± 0.24 | 1.06 ± 0.23c | 1.10 ± 0.21c |
P02760 | cDNA FLJ51445, highly similar to AMBP protein |
SKWn*ITMESYVVHTNYDEYAIFLTK | 0.83 ± 0.46 | 1.52 ± 0.88c | 1.71 ± 0.88c |
P00450 | Ceruloplasmin | EHEGAIYPDn*TTDFQR | 0.75 ± 0.32 | 1.10 ± 0.37c | 1.05 ± 0.30c |
En*LTAPGSDSAVFFEQGTTR | 0.78 ± 0.34 | 1.14 ± 0.42c | 1.12 ± 0.38c | ||
Q9NZP8 | Complement C1r subcomponent-like protein |
n*QSVNVFLGHTAIDEMLK | 0.90 ± 0.41 | 1.55 ± 0.64c | 1.38 ± 0.50c |
Q03591 | Complement factor H-related protein 2 | LQNNENn*ISCVER | 0.53 ± 0.27 | 1.40 ± 0.79c | 1.26 ± 0.97c |
P05156 | Complement factor I light chain | n*GTAVCATNR | 0.83 ± 0.26 | 1.36 ± 0.60c | 1.16 ± 0.45c |
Contactin-4 | SDVGn*YTCVVTNTVTNHK | 0.86 ± 0.25 | 1.06 ± 0.22 | 1.14 ± 0.18c | |
Q02487 | Desmocollin-2 | An*YTILK | 0.71 ± 0.33 | 0.91 ± 0.33 | 1.08 ± 0.22c |
Q9H4A9 | Dipeptidase 2 | GVHSFYNn*ISGLTDFGEK | 0.97 ± 0.19 | 1.25 ± 0.22c | 1.15 ± 0.20c |
Q14118 | Dystroglycan | LGAn* GSHIPQTSSVFSIEVYPEDHSELQSVR | 0.80 ± 0.23 | 1.19 ± 0.27c | 1.03 ± 0.17c |
P02790 | Hemopexin | ALPQPQn*VTSLLGCTH | 0.62 ± 0.25 | 1.18 ± 0.56c | 1.13 ± 0.34c |
SWPAVGn*CSSALR | 0.70 ± 0.38 | 1.04 ± 0.44c | 1.21 ± 0.55c | ||
P05546 | Heparin cofactor 2 | DFVn*ASSK | 0.71 ± 0.35 | 1.30 ± 0.50c | 1.16 ± 0.50c |
n*LSMPLLPADFHK | 0.71 ± 0.35 | 1.15 ± 0.40c | 1.07 ± 0.44c | ||
P26927 | Hepatocyte growth factor-like protein alpha chain |
GTGn*DTVLNVALLNVISNQECNIK | 0.86 ± 0.22 | 1.14 ± 0.56 | 1.24 ± 0.35c |
Q9Y6R7 | IgGFc-binding protein | VITVQVAn*FTLR | 0.74 ± 0.34 | 1.14 ± 0.89 | 1.39 ± 0.73c |
FDFQGTCEYLLSAPCHGPPLGAEn*FTVTVANEHR | 0.72 ± 0.35 | 1.25 ± 0.34c | 1.12 ± 0.39c | ||
YLPVn*SSLLTSDCSER | 0.79 ± 0.28 | 1.03 ± 0.60 | 1.23 ± 0.67c | ||
O14498 | Immunoglobulin superfamily containing leucine-rich repeat protein |
FQAFAn*GSLLIPDFGK | 0.78 ± 0.19 | 1.08 ± 0.26c | 1.04 ± 0.30c |
Q06033 | Interalpha-trypsin inhibitor heavy chain H3 |
NAHGEEKEn*LTAR | 0.54 ± 0.18 | 1.25 ± 0.74c | 1.10 ± 0.64c |
P40189 | Interleukin-6 receptor subunit beta | EQYTIIn*R(TA) | 0.82 ± 0.22 | 1.03 ± 0.20 | 1.13 ± 0.31c |
P01042 | Kininogen-1 | YNSQn*QSNNQFVLYR | 0.48 ± 0.37 | 2.12 ± 3.39c | 1.39 ± 0.84c |
P02750 | Leucine-rich alpha-2-glycoprotein | KLPPGLLAn*FTLLR | 0.68 ± 0.40 | 1.65 ± 1.32c | 1.49 ± 0.85a |
MFSQn*DTR | 0.66 ± 0.14 | 1.50 ± 1.08c | 1.02 ± 0.50c | ||
P51884 | Lumican | LSHNELADSGIPGNSFn*VSSLVELDLSYNK | 0.78 ± 0.21 | 1.04 ± 0.15c | 1.00 ± 0.17c |
P22897 | Macrophage mannose receptor 1 | WECKn*DTLLGIK | 0.88 ± 0.45 | 0.99 ± 0.32 | 1.37 ± 0.35c |
P01033 | Metalloproteinase inhibitor 1 | FVGTPEVn* QTTLYQR | 0.82 ± 0.25 | 1.14 ± 0.52 | 1.19 ± 0.38c |
Q92859 | Neogenin | TLSDVPSAAPQn*LSLEVR | 0.73 ± 0.15 | 1.09 ± 0.31c | 1.02 ± 0.16c |
P55058 | Phospholipid transfer protein | IYSn*HSALESLALIPLQAPLK | 0.82 ± 0.24 | 1.11 ± 0.40 | 1.05 ± 0.24c |
P05155 | Plasma protease C1 inhibitor | VLSn*NSDANLELINTWVAK | 0.90 ± 0.34 | 1.43 ± 0.69c | 1.33 ± 0.57c |
P01833 | Polymeric immunoglobulin receptor | VPGn*VTAVLGETLK | 0.62 ± 0.29 | 1.34 ± 0.84c | 1.15 ± 0.74c |
P41222 | Prostaglandin-H2 d-isomerase | WFSAGLASn*SSWLR | 0.88 ± 0.34 | 1.20 ± 0.93 | 1.58 ± 0.85c |
SVVAPATDGGLn*LTSTFLR | 0.82 ± 0.38 | 1.08 ± 0.87 | 1.29 ± 0.66c | ||
Q86VB7 | Scavenger receptor cysteine-rich type 1 protein M130 |
CKGn*ESSLWDCPAR | 0.74 ± 0.35 | 1.07 ± 0.49 | 1.10 ± 0.26c |
P01009 | Short peptide from AAT | ADTHDEILEGLNFn*LTEIPEAQIH | 0.50 ± 0.17 | 0.80 ± 0.55 | 0.90 ± 0.34c |
Thyroxine-binding globulin | VTACHSSQPn*ATLYK | 0.71 ± 0.33 | 1.08 ± 0.32c | 1.09 ± 0.58c | |
P00751 | Uncharacterized protein | SPYYn*VSDEISFHCYDGYTLR | 0.75 ± 0.39 | 1.22 ± 0.93 | 1.28 ± 0.53c |
P04004 | Vitronectin | Nn*ATVHEQVGGPSLTSDLQAQSK | 0.59 ± 0.46 | 2.70 ± 6.67 | 1.26 ± 0.79c |
P04275 | von Willebrand factor | GLQPTLTNPGECRPn*FTCACR | 0.71 ± 0.40 | 1.02 ± 0.98 | 1.44 ± 0.84c |
von Willebrand factor | MEACMLn*GTVIGPGK | 0.78 ± 0.22 | 1.03 ± 0.37 | 1.30 ± 0.79c | |
P25311 | Zinc-alpha-2-glycoprotein | FGCEIENn*R(SS) | 0.69 ± 0.28 | 1.48 ± 1.08 | 1.29 ± 0.43c |
DIYEYYn*DSNGSHVLQGR | 0.78 ± 0.21 | 1.02 ± 0.38 | 1.04 ± 0.37c | ||
P05154 | Plasma serine protease inhibitor | VLPSLGISNVFTSHADLSGISn*HSNIQVSEMVHK | 0.78 ± 0.42 | 1.02 ± 0.54 | 1.23 ± 0.39c |
Data represent the mean and SD value based on the reporter ion ratios for CF proteins quantified in at least 5 sample sets. Significant difference level P ≤ 0.05; N, healthy controls; CP, chronic pancreatitis; PC, pancreatic cancer; SR, standard reference.
Asterisk (*) indicates CF modification at asparagine (n) residue.
Difference between healthy controls and pancreatic cancer or chronic pancreatitis.