Table 1. Primers used in this study.
| Experiments | Primer | Sequences |
|---|---|---|
| Spo Cloning | F1 | CTATGGACGATGGAGCACCT |
| F2 | AACTGTGTCTGGGCTCTCGT | |
| F3 | GTCTGCATATCTAGGCTTACTACAGAGATA | |
| R1 | CGTCAGCCACATCTTTCTCA | |
| R2 | AGATAGCATGATAGCGAAGAAAGTCT | |
| R3 | CGTCAGCCACATCTTTCTCA | |
| R4 | TCTTGAAAGACGGCATCGTAGAGTC | |
| R5 | AGATAGCATGATAGCGAAGAAAGTCT | |
| Shd Cloning | F1 | GCAGATGAAGAACGTACAGACACT |
| F2 | CACAGCAAAAGTAAATTGGCTCTAC | |
| F3 | ACTGTGCTCCCAACAGGAAC | |
| F4 | AATAATGGATTTTATAGCAGGAGGAGT | |
| R1 | CGAGATACCTCAGCGTGACA | |
| R2 | GGTCTTGAATGTTGTCGATAGAATC | |
| R3 | CACTTTCCCCCACATCATTT | |
| R4 | GGAGGTCGTCACGGAATGTCT | |
| p IZT | F_Spe | GTGTGTACTAGTATGAGGCAGAGCTGCGGGCGGCTCGT |
| R_NotI | GTGTGTGCGGCCGCCGGAGGTCGTCACGGAATGTCT | |
| dsRNA/WISH | SpoF | AACTGTGTCTGGGCTCTCGT |
| SpoR | AGGTGCTCCATCGTCCATAG | |
| dsRNA | ShdF | TCGGAACACTTTGCGATGTA |
| ShdR | ACTGTAGGGCTGAGCCTGAA | |
| dsRNA | GFPF | ATGAGTAAAGGAGAAGAACTTTT |
| GFPR | TTTGTATAGTTCATCCATGCCA | |
| qRT-PCR | ActF | ATGGTGGGTATGGGTCAGAA |
| ActR | AGGTGTGGTGCCAGATCTTC | |
| Spo_RealF | TTCTCGGAAAGACTGCCAAT | |
| Spo_RealR | GAGGGTGCAGCCAACTATGT | |
| Shd_RealF | ACCGGTTCACTCTGGAATCA | |
| Shd_RealR | GCCCATAGTACAGCCTCTGG |