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. 2014 Dec 23;1:140046. doi: 10.1038/sdata.2014.46

Table 8. Elution-Test Dataset Imaging Log.

Ribbon Imaging Session Imaging Date primary ab antigen primary ab raised in primary ab vendor primary ab concentration secondary ab fluorophor secondary ab against secondary ab raised in secondary ab concentration exposure (ms) notes/caveats
This table contains a log of all stains/imaging sessions included in the Elution-Test Dataset (Data Citation 1, Files 1–7 Supplementary Table 1). All secondary antibodies raised in goat and applied at a concentration of 1:150.                        
Ex1-R02-A 1 Synapsin1 Rb Cell Signaling 1:300 Alexa 647 Rb Gt 1:150 592
NR2B Ms Millipore 1:500 Alexa 594 Ms Gt 1:150 667
vGluT2 GP Millipore 1:5,000 Alexa 488 GP Gt 1:150 788
2 Synapsin1 Rb Cell Signaling 1:300 Alexa 647 Rb Gt 1:150 592
NR2B Ms Millipore 1:500 Alexa 594 Ms Gt 1:150 667 saturated (would now image at 274 ms)
vGluT2 GP Millipore 1:5,000 Alexa 488 GP Gt 1:150 788
3 Synapsin1 Rb Cell Signaling 1:300 Alexa 647 Rb Gt 1:150 592 somewhat saturated (would now image at 459 ms)
NR2B Ms Millipore 1:500 Alexa 594 Ms Gt 1:150 667 saturated (would now image at 235 ms)
vGluT2 GP Millipore 1:5,000 Alexa 488 GP Gt 1:150 788 somewhat saturated (would now image at 575 ms)
Ex1-R07-A 1 3/6/2014 PSD95 Rb Cell Signaling 1:300 Alexa 647 Rb Gt 1:150 2,050
NR2A Ms Millipore 1:50 Alexa 594 Ms Gt 1:150 1,160
vGluT1 GP Millipore 1:5,000 Alexa 488 GP Gt 1:150 843
2 4/6/2014 PSD95 Rb Cell Signaling 1:300 Alexa 647 Rb Gt 1:150 2,050
NR2A Ms Millipore 1:50 Alexa 594 Ms Gt 1:150 1,160
vGluT1 GP Millipore 1:5,000 Alexa 488 GP Gt 1:150 843
3 4/6/2014 PSD95 Rb Cell Signaling 1:300 Alexa 647 Rb Gt 1:150 2,050
NR2A Ms Millipore 1:50 Alexa 594 Ms Gt 1:150 1,160
vGluT1 GP Millipore 1:5,000 Alexa 488 GP Gt 1:150 843
Ex1-R07-B 1 3/6/2014 GAD2 Rb Cell Signaling 1:200 Alexa 488 Rb Gt 1:150 1,180
vGAT Ms Synaptic Systems 1:100 Alexa 594 Ms Gt 1:150 1,090
2 4/6/2014 GAD2 Rb Cell Signaling 1:200 Alexa 488 Rb Gt 1:150 1,180
vGAT Ms Synaptic Systems 1:100 Alexa 594 Ms Gt 1:150 1,090 vGAT very messy
3 4/6/2014 GAD2 Rb Cell Signaling 1:200 Alexa 488 Rb Gt 1:150 1,180
vGAT Ms Synaptic Systems 1:100 Alexa 594 Ms Gt 1:150 1,090
Ex1-R07-C 1 3/6/2014 PV25 Rb Swant 1:100 Alexa 647 Rb Gt 1:150 876
Gephyrin Ms BD Biosciences 1:300 Alexa 594 Ms Gt 1:150 964
2 4/6/2014 PV25 Rb Swant 1:100 Alexa 647 Rb Gt 1:150 876 PV ubiquitous
Gephyrin Ms BD Biosciences 1:300 Alexa 594 Ms Gt 1:150 964
3 4/6/2014 PV25 Rb Swant 1:100 Alexa 647 Rb Gt 1:150 876
Gephyrin Ms BD Biosciences 1:300 Alexa 594 Ms Gt 1:150 964
Ex1-R08-D 1 3/6/2014 GluR1 Rb Millipore 1:100 Alexa 488 Rb Gt 1:150 800 background
GluR2 Ms Millipore 1:50 Alexa 594 Ms Gt 1:150 761
2 4/6/2014 GluR1 Rb Millipore 1:100 Alexa 488 Rb Gt 1:150 800
GluR2 Ms Millipore 1:50 Alexa 594 Ms Gt 1:150 761
3 4/6/2014 GluR1 Rb Millipore 1:100 Alexa 488 Rb Gt 1:150 800
GluR2 Ms Millipore 1:50 Alexa 594 Ms Gt 1:150 761
Ex1-R08-E 1 3/6/2014 Synaptopodin Rb Synaptic Systems 1:500 Alexa 647 Rb Gt 1:150 1,430
GABAARa1 Ms NeuroMABs 1:100 Alexa 594 Ms Gt 1:150 2,240 almost no signal
2 4/6/2014 Synaptopodin Rb Synaptic Systems 1:500 Alexa 647 Rb Gt 1:150 1,430
GABAARa1 Ms NeuroMABs 1:100 Alexa 594 Ms Gt 1:150 2,240
3 4/6/2014 Synaptopodin Rb Synaptic Systems 1:500 Alexa 647 Rb Gt 1:150 1,430
GABAARa1 Ms NeuroMABs 1:100 Alexa 594 Ms Gt 1:150 2,240
Ex1-R08-F 1 3/6/2014 Arc GP Synaptic Systems 1:150 Alexa 647 Rb Gt 1:150 2,750
GluR4 Rb Cell Signaling 1:50 Alexa 488 Ms Gt 1:150 1,370
2 4/6/2014 Arc GP Synaptic Systems 1:150 Alexa 647 Rb Gt 1:150 2,750 Arc very saturated
GluR4 Rb Cell Signaling 1:50 Alexa 488 Ms Gt 1:150 1,370
3 4/6/2014 Arc GP Synaptic Systems 1:150 Alexa 647 Rb Gt 1:150 2,750
GluR4 Rb Cell Signaling 1:50 Alexa 488 Ms Gt 1:150 1,370