Table 9. Antibody Colocalization.
| Correlation Peak (r) | Synapsin1 | vGluT1 | vGluT2 | PSD95 | GluR1 | GluR2 | NR2A | NR2B | GAD2 | vGAT | Gephyrin | GABAARa1 | PV25 | DAPI |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| We performed a pairwise 2-dimensional cross-correlation analysis to quantify spatial correlations (colocalization) between the principal antibodies used in these experiments This table displays 2-d cross-correlation peak, half-max contour area, and equivalent circular diameter values for each pairwise combination of antibodies. (See Technical Validation: Antibody Specificity; Figure 3). | ||||||||||||||
| Synapsin1 | 0.394 | 0.127 | 0.025 | 0.033 | 0.017 | 0.024 | 0.000 | 0.013 | 0.050 | 0.018 | 0.002 | 0.007 | −0.003 | −0.012 |
| vGluT1 | 0.127 | 0.394 | 0.026 | 0.020 | 0.001 | 0.016 | 0.002 | 0.025 | 0.033 | 0.000 | 0.000 | 0.004 | 0.005 | 0.001 |
| vGluT2 | 0.025 | 0.026 | 0.394 | 0.011 | 0.000 | 0.006 | 0.005 | 0.004 | −0.002 | 0.002 | 0.032 | 0.008 | 0.013 | 0.017 |
| PSD95 | 0.033 | 0.020 | 0.011 | 0.394 | −0.002 | 0.079 | 0.011 | 0.043 | 0.003 | −0.005 | 0.001 | 0.004 | −0.005 | −0.006 |
| GluR1 | 0.017 | 0.001 | 0.000 | −0.002 | 0.394 | 0.018 | 0.041 | 0.051 | 0.074 | 0.148 | 0.056 | 0.075 | 0.020 | 0.003 |
| GluR2 | 0.024 | 0.016 | 0.006 | 0.079 | 0.018 | 0.394 | 0.016 | 0.033 | 0.015 | 0.017 | 0.018 | 0.033 | 0.004 | 0.018 |
| NR2A | 0.000 | 0.002 | 0.005 | 0.011 | 0.041 | 0.016 | 0.394 | 0.038 | 0.006 | 0.038 | 0.076 | 0.066 | 0.001 | 0.001 |
| NR2B | 0.013 | 0.025 | 0.004 | 0.043 | 0.051 | 0.033 | 0.038 | 0.394 | 0.010 | 0.036 | 0.042 | 0.066 | −0.001 | −0.001 |
| GAD2 | 0.050 | 0.033 | −0.002 | 0.003 | 0.074 | 0.015 | 0.006 | 0.010 | 0.394 | 0.079 | 0.018 | 0.031 | 0.009 | 0.005 |
| vGAT | 0.018 | 0.000 | 0.002 | −0.005 | 0.148 | 0.017 | 0.038 | 0.036 | 0.079 | 0.394 | 0.054 | 0.079 | 0.029 | 0.004 |
| Gephyrin | 0.002 | 0.000 | 0.032 | 0.001 | 0.056 | 0.018 | 0.076 | 0.042 | 0.018 | 0.054 | 0.394 | 0.091 | 0.002 | 0.001 |
| GABAARa1 | 0.007 | 0.004 | 0.008 | 0.004 | 0.075 | 0.033 | 0.066 | 0.066 | 0.031 | 0.079 | 0.091 | 0.394 | 0.014 | 0.013 |
| PV25 | −0.003 | 0.005 | 0.013 | −0.005 | 0.020 | 0.004 | 0.001 | −0.001 | 0.009 | 0.029 | 0.002 | 0.014 | 0.394 | 0.012 |
| DAPI | −0.012 | 0.001 | 0.017 | −0.006 | 0.003 | 0.018 | 0.001 | −0.001 | 0.005 | 0.004 | 0.001 | 0.013 | 0.012 | 0.394 |
|
Contour Area (px^2)
|
||||||||||||||
| Synapsin1 | 5.77 | 9.13 | 8.82 | 19.78 | 9.22 | 14.59 | 125.38 | 15.25 | 8.81 | 9.57 | 16.48 | 13.69 | 318.64 | 156.17 |
| vGluT1 | 9.12 | 5.69 | 7.63 | 21.81 | 21.50 | 16.59 | 11.57 | 9.49 | 6.96 | 8.37 | 352.89 | 13.80 | 5.12 | 21.95 |
| vGluT2 | 8.76 | 7.63 | 5.36 | 9.61 | 388.84 | 10.56 | 11.79 | 10.71 | 30.38 | 131.79 | 7.87 | 12.46 | 9.54 | 36.26 |
| PSD95 | 19.71 | 21.76 | 9.61 | 3.96 | 35.87 | 6.72 | 7.04 | 6.47 | 14.42 | 20.83 | 28.25 | 7.22 | 21.55 | 38.20 |
| GluR1 | 9.17 | 62.60 | 219.17 | 35.79 | 5.07 | 14.35 | 11.85 | 8.70 | 10.32 | 8.11 | 14.38 | 11.88 | 12.06 | 397.91 |
| GluR2 | 14.54 | 16.54 | 10.53 | 6.72 | 14.37 | 4.80 | 15.18 | 9.18 | 14.41 | 13.98 | 15.45 | 13.44 | 20.89 | 32.03 |
| NR2A | 82.12 | 10.79 | 11.85 | 7.03 | 11.88 | 15.11 | 2.73 | 11.00 | 24.73 | 11.37 | 8.55 | 12.00 | 15.47 | 67.40 |
| NR2B | 15.11 | 9.46 | 10.72 | 6.46 | 8.69 | 9.17 | 11.02 | 4.03 | 18.66 | 10.05 | 12.94 | 10.21 | 455.36 | 247.07 |
| GAD2 | 8.77 | 6.93 | 29.41 | 1.75 | 10.34 | 14.33 | 24.84 | 18.63 | 5.31 | 10.64 | 22.54 | 15.21 | 12.65 | 30.15 |
| vGAT | 9.51 | 208.44 | 14.48 | 20.92 | 8.12 | 13.90 | 11.36 | 10.05 | 10.62 | 5.17 | 14.68 | 11.53 | 11.63 | 160.51 |
| Gephyrin | 15.86 | 251.98 | 7.86 | 59.59 | 14.38 | 15.40 | 8.53 | 12.91 | 22.43 | 14.65 | 5.00 | 11.68 | 38.20 | 101.67 |
| GABAARa1 | 13.57 | 13.19 | 12.57 | 7.17 | 11.86 | 13.36 | 11.98 | 10.18 | 15.16 | 11.52 | 11.70 | 5.42 | 13.70 | 114.35 |
| PV25 | 286.11 | 5.03 | 9.57 | 21.89 | 12.09 | 20.39 | 114.91 | 69.03 | 12.68 | 11.65 | 37.09 | 13.77 | 5.18 | 72.26 |
| DAPI | 145.74 | 22.59 | 37.12 | 35.12 | 288.63 | 32.59 | 78.27 | 321.33 | 29.96 | 304.59 | 26.77 | 116.32 | 73.49 | 18.00 |
|
Contour Diameter (px)
|
||||||||||||||
| Synapsin1 | 2.71 | 3.41 | 3.35 | 5.02 | 3.43 | 4.31 | 12.64 | 4.41 | 3.35 | 3.49 | 4.58 | 4.18 | 20.14 | 14.10 |
| vGluT1 | 3.41 | 2.69 | 3.12 | 5.27 | 5.23 | 4.60 | 3.84 | 3.48 | 2.98 | 3.26 | 21.20 | 4.19 | 2.55 | 5.29 |
| vGluT2 | 3.34 | 3.12 | 2.61 | 3.50 | 22.25 | 3.67 | 3.87 | 3.69 | 6.22 | 12.95 | 3.17 | 3.98 | 3.49 | 6.79 |
| PSD95 | 5.01 | 5.26 | 3.50 | 2.25 | 6.76 | 2.92 | 2.99 | 2.87 | 4.28 | 5.15 | 6.00 | 3.03 | 5.24 | 6.97 |
| GluR1 | 3.42 | 8.93 | 16.71 | 6.75 | 2.54 | 4.27 | 3.88 | 3.33 | 3.63 | 3.21 | 4.28 | 3.89 | 3.92 | 22.51 |
| GluR2 | 4.30 | 4.59 | 3.66 | 2.93 | 4.28 | 2.47 | 4.40 | 3.42 | 4.28 | 4.22 | 4.43 | 4.14 | 5.16 | 6.39 |
| NR2A | 10.23 | 3.71 | 3.88 | 2.99 | 3.89 | 4.39 | 1.87 | 3.74 | 5.61 | 3.81 | 3.30 | 3.91 | 4.44 | 9.26 |
| NR2B | 4.39 | 3.47 | 3.70 | 2.87 | 3.33 | 3.42 | 3.75 | 2.27 | 4.87 | 3.58 | 4.06 | 3.60 | 24.08 | 17.74 |
| GAD2 | 3.34 | 2.97 | 6.12 | 1.49 | 3.63 | 4.27 | 5.62 | 4.87 | 2.60 | 3.68 | 5.36 | 4.40 | 4.01 | 6.20 |
| vGAT | 3.48 | 16.29 | 4.29 | 5.16 | 3.21 | 4.21 | 3.80 | 3.58 | 3.68 | 2.56 | 4.32 | 3.83 | 3.85 | 14.30 |
| Gephyrin | 4.49 | 17.91 | 3.16 | 8.71 | 4.28 | 4.43 | 3.30 | 4.06 | 5.34 | 4.32 | 2.52 | 3.86 | 6.97 | 11.38 |
| GABAARa1 | 4.16 | 4.10 | 4.00 | 3.02 | 3.89 | 4.12 | 3.91 | 3.60 | 4.39 | 3.83 | 3.86 | 2.63 | 4.18 | 12.07 |
| PV25 | 19.09 | 2.53 | 3.49 | 5.28 | 3.92 | 5.10 | 12.10 | 9.38 | 4.02 | 3.85 | 6.87 | 4.19 | 2.57 | 9.59 |
| DAPI | 13.62 | 5.36 | 6.87 | 6.69 | 19.17 | 6.44 | 9.98 | 20.23 | 6.18 | 19.69 | 5.84 | 12.17 | 9.67 | 4.79 |