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. 2015 Apr 22;10:3065–3080. doi: 10.2147/IJN.S82199

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© 2015 Nurhasni et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Confocal microscopy images and the percent (%) survival of MRSA (left panel) and Pseudomonas aeruginosa (right panel) after 24 hours of treatment with nanoparticles at different concentrations.

Notes: (A) PPNPs, (B) NO/PPNPs, (C) percent (%) survival against MRSA and (D) percent (%) survival against P. aeruginosa. Syto-9 fluorescence (green) indicates intact membrane of healthy bacteria, PI fluorescence (red) indicates membrane destruction and cell death. Bacterial survival at each point is presented as a percentage relative to the control group (buffer alone). Data shown are mean ± standard deviation; n=3. (A and B) Bars represent 20 μm.

Abbreviations: PLGA, poly(lactic-co-glycolic acid); PEI, polyethylenimine; PPNPs, PLGA-PEI nanoparticles; NO/PPNPs, NO-releasing PLGA-PEI nanoparticles; MRSA, methicillin-resistant Staphylococcus aureus; PI, propidium iodide; NO, nitric oxide.

Confocal microscopy images and the percent (%) survival of MRSA (left panel) and Pseudomonas aeruginosa (right panel) after 24 hours of treatment with nanoparticles at different concentrations.

Notes: (A) PPNPs, (B) NO/PPNPs, (C) percent (%) survival against MRSA and (D) percent (%) survival against P. aeruginosa. Syto-9 fluorescence (green) indicates intact membrane of healthy bacteria, PI fluorescence (red) indicates membrane destruction and cell death. Bacterial survival at each point is presented as a percentage relative to the control group (buffer alone). Data shown are mean ± standard deviation; n=3. (A and B) Bars represent 20 μm.

Abbreviations: PLGA, poly(lactic-co-glycolic acid); PEI, polyethylenimine; PPNPs, PLGA-PEI nanoparticles; NO/PPNPs, NO-releasing PLGA-PEI nanoparticles; MRSA, methicillin-resistant Staphylococcus aureus; PI, propidium iodide; NO, nitric oxide.