Table 3. Stability of reference gene expression under abiotic conditions.
Abiotic conditions | Reference gene | geNorm | Normfider | BestKeeper | ΔCt | ||||
---|---|---|---|---|---|---|---|---|---|
Stability | Rank | Stability | Rank | Stability | Rank | Stability | Rank | ||
Temperature | 28S | 0.19 | 1 | 0.15 | 3 | 0.17 | 1 | 0.38 | 2 |
CypA | 0.35 | 5 | 0.39 | 5 | 0.35 | 5 | 0.53 | 5 | |
EF1A | 0.19 | 1 | 0.10 | 1 | 0.19 | 2 | 0.37 | 1 | |
ATPase | 0.21 | 2 | 0.14 | 2 | 0.19 | 2 | 0.39 | 3 | |
GAPDH | 0.50 | 6 | 0.83 | 7 | 0.59 | 6 | 0.87 | 7 | |
18S | 0.22 | 3 | 0.18 | 4 | 0.26 | 3 | 0.41 | 4 | |
Actin | 0.30 | 4 | 0.44 | 6 | 0.33 | 4 | 0.55 | 6 | |
Photoperiod | 28S | 1.44 | 3 | 1.50 | 4 | 0.47 | 1 | 2.57 | 4 |
CypA | 1.30 | 2 | 0.66 | 2 | 1.77 | 5 | 2.35 | 2 | |
EF1A | 2.25 | 5 | 3.51 | 6 | 1.53 | 4 | 3.73 | 6 | |
ATPase | 1.15 | 1 | 0.78 | 3 | 1.50 | 3 | 2.39 | 3 | |
GAPDH | 1.15 | 1 | 0.57 | 1 | 0.79 | 2 | 2.25 | 1 | |
18S | 3.04 | 6 | 1.50 | 7 | 5.11 | 7 | 4.99 | 7 | |
Actin | 1.87 | 4 | 1.75 | 5 | 2.54 | 6 | 2.96 | 5 | |
28S | 0.43 | 5 | 0.48 | 6 | 0.38 | 6 | 0.57 | 6 | |
CypA | 0.48 | 6 | 0.53 | 7 | 0.44 | 7 | 0.60 | 7 | |
dsRNA | 28S | 0.45 | 3 | 0.42 | 3 | 0.46 | 5 | 0.65 | 3 |
CypA | 0.28 | 2 | 0.21 | 1 | 0.35 | 1 | 0.60 | 1 | |
EF1A | 0.53 | 5 | 0.52 | 6 | 0.72 | 6 | 0.75 | 6 | |
ATPase | 0.76 | 6 | 1.29 | 7 | 1.27 | 7 | 1.35 | 7 | |
GAPDH | 0.25 | 1 | 0.44 | 4 | 0.44 | 2 | 0.68 | 5 | |
18S | 0.48 | 4 | 0.48 | 5 | 0.45 | 3 | 0.68 | 4 | |
Actin | 0.25 | 1 | 0.22 | 2 | 0.45 | 4 | 0.62 | 2 |