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. 2015 Apr 27;10(4):e0125868. doi: 10.1371/journal.pone.0125868

Table 3. Stability of reference gene expression under abiotic conditions.

Abiotic conditions Reference gene geNorm Normfider BestKeeper ΔCt
Stability Rank Stability Rank Stability Rank Stability Rank
Temperature 28S 0.19 1 0.15 3 0.17 1 0.38 2
CypA 0.35 5 0.39 5 0.35 5 0.53 5
EF1A 0.19 1 0.10 1 0.19 2 0.37 1
ATPase 0.21 2 0.14 2 0.19 2 0.39 3
GAPDH 0.50 6 0.83 7 0.59 6 0.87 7
18S 0.22 3 0.18 4 0.26 3 0.41 4
Actin 0.30 4 0.44 6 0.33 4 0.55 6
Photoperiod 28S 1.44 3 1.50 4 0.47 1 2.57 4
CypA 1.30 2 0.66 2 1.77 5 2.35 2
EF1A 2.25 5 3.51 6 1.53 4 3.73 6
ATPase 1.15 1 0.78 3 1.50 3 2.39 3
GAPDH 1.15 1 0.57 1 0.79 2 2.25 1
18S 3.04 6 1.50 7 5.11 7 4.99 7
Actin 1.87 4 1.75 5 2.54 6 2.96 5
28S 0.43 5 0.48 6 0.38 6 0.57 6
CypA 0.48 6 0.53 7 0.44 7 0.60 7
dsRNA 28S 0.45 3 0.42 3 0.46 5 0.65 3
CypA 0.28 2 0.21 1 0.35 1 0.60 1
EF1A 0.53 5 0.52 6 0.72 6 0.75 6
ATPase 0.76 6 1.29 7 1.27 7 1.35 7
GAPDH 0.25 1 0.44 4 0.44 2 0.68 5
18S 0.48 4 0.48 5 0.45 3 0.68 4
Actin 0.25 1 0.22 2 0.45 4 0.62 2