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. 2015 Apr 27;10(4):e0126691. doi: 10.1371/journal.pone.0126691

Fig 1. Cytotoxic effect of Eltrombopag on AML cells is dependent on dose and serum concentration.

Fig 1

A) Dose response of primary AML cells and MOLM14 AML cell line (left panel) to E in culture medium containing 2% FBS and response to 5 μM E at the increasing concentration of serum in culture medium (2–10%) (right panel). Graphs represent cell counts at Day 4 for primary AML sample and Day 2 for MOLM 14 cells. Total number of viable cells in treated samples is presented as a % of total number of viable cells in control samples (untreated cells). Data are presented as an average of 3 independent experiments with a standard error of the mean (SEM). B) Time course of the response to 5 μM E in representative primary AML sample and MOLM14 cell line in 2% serum. C) Summary of the E’s effect on human primary AML cells n = 9 compared to peripheral blood mononuclear cells (PB-MNCs) from normal donors (ND) n = 3 (left panel) and AML cell lines n = 4 (right panel). Graphs demonstrate decrease in a total cell number after exposure to 5 μM E at 2% serum, presented as a percent of control at day 2 for cell lines and day 4 for primary cells.