Fig 3. The role of AaHig in flaviviruses infection of A. aegypti.

Immuno-blockade of AaHig enhanced the DENV-2 (A-B) and JEV (C-D) infections in the whole bodies (i) and heads (ii) of mosquitoes. The murine AaHig antibody, in the 10-fold serial dilutions, was premixed with 10 M.I.D.₅₀ viruses to co-microinject into the thorax of mosquitoes. The treated mosquitoes were sacrificed to examine the viral load in the whole mosquito bodies (i) and heads (ii) at 3 (A, C) and 6 (B, D) days post-infection by TaqMan qPCR and normalized against A. aegypti actin. The results were reproduced by 3 times. One dot represents 1 mosquito/head and the horizontal line represents the median of the results. The data were analyzed statistically using the non-parametric Mann-Whitney test.