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. 2015 Apr 27;10(4):e0125405. doi: 10.1371/journal.pone.0125405

Fig 2. Protein expression of candidate biomarkers in human cell lines.

Fig 2

(A) Total protein levels of CREB-binding protein (CREBBP) and CD81 were analyzed by western blotting. β-Tubulin was used as a calibration control. (B) The expression of CREBBP and CD81 were examined by immunofluorescence assay. CREBBP expression was mainly detected in the nucleus, and its expression was higher in T24 cells than in primary human urothelial cells. CD81 expression was prominent at the cell surface of primary human urothelial cells, but was only detected in a few T24 cells. Representative images from 3 independent experiments are shown. Nuclei and target proteins were detected using immunofluorescence staining and observed with a Nikon Eclipse E400 microscope at 400× magnification. UC, primary human urothelial cells.