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. 2015 Apr 27;10(4):e0122270. doi: 10.1371/journal.pone.0122270

Fig 3. Blocking ER stress inhibits PA induced apoptosis.

Fig 3

(A) PANC-1 and (B) MIA PaCa-2 cells were treated with or without PA (30 μM) in presence of TUDCA (200 μM) for 24 h. Apoptosis was detected by Cell Death Detection ELISA and expressed relative to vehicle-treated cells (set equal to 1). Data were compared by ANOVA with Bonferroni correction for the significant level. Here, *p≤0.025 was considered significant for the individual t-tests. Apoptosis was confirmed in C) PANC-1 and D) MIA PaCa-2 cells treated with same concentrations of PA in presence of TUDCA (200 μM) for 24 h. Representative blots show expression of PARP cleavage (c-PARP) and β-actin was used as loading control. Effect of TUDCA on PA induced expressions of ATF4 and CHOP in (C) PANC-1 and (D) MIA PaCa-2 cells were shown as well. Three independent experiments were done for the western blot studies and quantitative data composed of all the experiments in E) PANC-1 and F) MIA PaCa-2 cells with statistical analysis were shown below the representative blot image. *P<0.050 was considered to be significant when compared to control (n = 3) by Student t-test. The graphical data represent mean +/- SD.