Fig 3. p110α regulates LIFR expression through c-Myc.

The protein expression levels of c-Myc and LIFRα were evaluated after 12h, 24h and 30h of 100nM PIK75 exposure by Western blot (a) in DAOY-derived cell clones overexpressing c-Myc (DAOY M2.1) and (b) in UW228-Myc-ER cells induced for c-Myc expression by tamoxifen for 24 h, 48 h and 72 h. (c) LIFRα mRNA levels were analyzed by qPCR and protein levels were analyzed by Western blot in the inducible UW228-Myc-ER cells treated independently, simultaneously or in alternate manner with the p110α inhibitor PIK75 (100 nM) and/or with tamoxifen (500 nM) for 24 h or 48 h. UW228-Myc-ER were simultaneously exposed to both drugs for 24h (bar 4/lane 4), alternately for 48 h, bar 4/lane 4 24 h tamoxifen (500 nM) exposure followed by 24 h PIK75 (100 nM) and bar 5/lane5 24 h PIK75 (100 nM) treatment followed by 24h tamoxifen (500 nM) induction.