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. 2015 Apr 27;10(4):e0123958. doi: 10.1371/journal.pone.0123958

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© 2015 Salm et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — Expression of c-Myc (a) and LIFRα (b) were analyzed by qPCR and Western Blot in DAOY M2.1 cells, overexpressing c-Myc, transfected with siRNA against c-Myc. (c) miR-125b expression levels were measured by qPCR after 24h and 48h of 4-OHT treatment in UW228-Myc-ER cells. MiR-125b fold change was calculated via normalization to U6 snRNA expression levels in the corresponding condition (d) miR-125b expression was analyzed by qPCR after transfection with mimic or antagomir for miR-125b in DAOY cells. MiR-125b fold change was calculated as in (c) (e) LIFRα expression level was analyzed by qPCR and Western blot after transfection with mimic or antagomir for miR-125b. (b) and (e) were quantified by ImageJ analysis software.