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. 2015 Apr 27;10(4):e0123958. doi: 10.1371/journal.pone.0123958

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© 2015 Salm et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — (a) Protein expression level of LIFRα in DAOY cells transfected with siRNA against LIFR compared to the control siRNA transfected cells. (b) The effects on cell viability of the siRNA-mediated target down-regulation of LIFRα (white bars) compared to control siRNA (black bars) in DAOY and UW228 cells was assessed for the indicated time points and expressed as percentages. (c) The protein expression of LIFRα downstream targets was analyzed upon treatment for 10 or 30 min with 20μg/ml of anti-LIFRα neutralizing antibody. β-actin was used as a loading control. (d) Cell viability was evaluated in DAOY, UW228, PNET5 and PFSK cells treated for 48h with an anti-LIFRα neutralizing antibody at the indicated concentrations.