Fig 4. A mediator is required in order to produce photocurrent with tobacco thylakoids.
A. Syn thylakoids transfer electrons directly to the electrode (black, no mediator added). The addition of DCBQ (red) resulted in inhibition of the photocurrent. Direct and DCBQ-mediated photocurrents produced by thylakoid of WT (dashed lines) or K238E (solid lines) strains of Syn were measured on a graphite-working electrode under external bias of 0.05VAg/AgCl and 0.24VAg/AgCl, respectively. The inset illustrates the schematic representation of the electron abstraction pathways from the Syn thylakoids. Thin and thick arrows indicate the magnitude of electron transfer, respectively. B. Unmediated (orange) and DCBQ-mediated (blue) photocurrent obtained from WT-tobacco (dashed lines) or R238E-tobacco (full lines) as described in panel A. Inset illustrated the electron abstraction pathways from the tobacco lines.