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. 2015 Apr 27;10(4):e0124839. doi: 10.1371/journal.pone.0124839

Fig 3. Binding of HbAHP-25 to recombinant gp120 protein.

Fig 3

Binding of HbAHP-25 or scrambled peptide (sP) to gp120 of HIV-1 IIIB (a) and HIV-1 BaL (b). Wells coated with various concentrations of HbAHP-25 or scrambled peptide (sP) were incubated with gp120 for 2 hrs at 37°C. This was followed by addition of gp120 antibody and absorbance was read at 492 nm. Background absorbance was subtracted from the wells where only gp120 was added (no HbAHP-25). Each bar represents the mean (± SD) of six observations from three experiments performed on different days (*p<0.05; **p<0.01; ***p<0.001).