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. 2015 Apr 27;10(4):e0124839. doi: 10.1371/journal.pone.0124839

Fig 5. Binding of HbAHP-25 to cellular gp120.

Fig 5

Offset histograms of cells (a) HL2/3 cells (b) TZM-bl cells and (c) HeLa cells indicate the extent of fluorescence intensity observed in FL-1A channel. Cells were treated with HbAHP-25 for 1 hr at RT, and then incubated with anti-HbAHP-25 antibody for 30 min. This was followed by addition of FITC labeled goat anti-rat antibody. Red lines correspond to the fluorescence of untreated group i.e only cells. The black lines correspond to the primary antibody control, while as blue lines correspond to the secondary antibody (FITC labeled) control, where both primary and secondary antibody was added to cells. The green line is the test group, and signifies the fluorescence in the peptide-treated group. The figure is representative image of one of the three independent experiments. (d, e) Expression of gp120 on HL2/3 cells (e). HL2/3 cells were treated with 2G12 mAb (500ng/ml), followed by anti-human (FITC labeled) secondary antibody. Cells not treated with 2G12 were used as a negative control (d).