Fig 9. Effect of HbAHP-25 on different stages of HIV-1 infection.

TZM-bl cells were infected with HIV-1 strains (IIIB) (a) and (Ada-5) (b) and HbAHP-25 was added during different stages of infection. (Pre: Viral strains pre-treated with the peptide were used for infecting TZM-bl cells; During: TZM-bl cells were simultaneously treated with viral strains and peptide; Post: TZM-bl cells were infected with HIV-1 virus first and 6 hrs later treated with the peptide and; all steps: HbAHP-25 was added at all the three steps. Luciferase activity was measured after 48 hrs of infection and per cent inhibition was calculated as mentioned earlier. sP and VRC01 were used as negative and positive controls respectively. Values are the mean (± SD) of six observations from three experiments performed on different days (**p<0.01; ***p<0.001).