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. 2015 Apr 27;10(4):e0124263. doi: 10.1371/journal.pone.0124263

Fig 4. Rv2463 upregulates CACNA1S in a TLR dependent and MyD88 independent pathway on macrophages.

Fig 4

J774 cells were transfected with siRNAs against MyD88, TRAF6 or IRAK1 for 36h followed by stimulations with 25 μg/ml Rv2463 or infection with 2 MOI of M. tb H37Rv for 48h. Surface CACNA1S expression was monitored by flow cytometry. Grey lines represent cells transfected with control siRNAs followed by stimulations with Rv2463 or infection with M. tb H37Rv, while bold lines represent cells transfected with specific siRNAs to indicated molecules followed by stimulations with Rv2463 or infection with M. tb H37Rv. Dotted line represents unstimulated or uninfected cells transfected with control siRNAs. One of three independent experiments is shown. P<0.015, control siRNA+Rv2463 v/s siMyD88+Rv2463; P<0.005, control siRNA+Rv2463 v/s siTRAF6+Rv2463; P<0.009, control siRNA+Rv2463 v/s siIRAK1+Rv2463. P values were calculated for 48h time point. Two-tailed Student’s t-test was employed for P values.