Fig 1. ALDH1^high cells were detected in the RD and KYM-1 cells.

RD (A) and KYM-1 (B) cells were stained with DEAB (control: left panel) or without DEAB (right panel) after being stained with BAAA and then analyzed using FACS Aria II with the ALUDEFLUOR assay kit. The proportion of ALDH1^high cells was 3.9±0.26% in the RD cells and 8.2±0.14% in the KYM-1 cells. The mean ± SD was calculated from three independent experiments. All ALDH1^high cells and a subset of the ALDH1^low cells were sorted as shown in the right panels of Fig 1A and 1B.