Table 1.

Definitions of parameters used within the IncuCyte ZOOM software.

Parameter	Function
RCU threshold	A fixed threshold level (in red calibrated fluorescence units) is used across the image, so all objects below this level are classified as background and not included in calculations
Edge sensitivity	Edge split sensitivity determines whether closely-spaced objects are separated, according to the weak signal points between them. A larger edge sensitivity number (i.e., 0 compared to −30) results in more splits, producing a higher count
Area filter	This function eliminates fluorescent objects that are outside the chosen size (in μm2)